马流产沙门菌鞭毛蛋白FliC对马腺疫链球菌SeM蛋白免疫效应分析

doi:10.11843/j.issn.0366-6964.2018.05.017

摘要/Abstract

摘要：

为研究马流产沙门菌（Salmonella abortus equi）鞭毛蛋白FliC对马腺疫链球菌（Streptococcus equi subsp.equi）SeM蛋白免疫效果的增强效应，为马腺疫新型高效疫苗的研发奠定基础。本研究构建并诱导表达了FliC，经亲和层析纯化目的蛋白，Western blot检测其免疫原性。将该纯化后FliC与SeM蛋白联合免疫C57BL/6小鼠，免疫后对小鼠进行抗体水平、抗体类型和攻毒保护力的检测。结果表明，成功表达、纯化了FliC重组蛋白，SDS-PAGE电泳检测显示该蛋白质为52 ku。抗体分型检测结果显示产生的抗体主要以IgG1为主；免疫30 d后FliC+SeM免疫组的攻毒保护率为87%，高于SeM免疫组的保护率67%。马流产沙门菌来源的鞭毛蛋白FliC能够增强SeM蛋白的免疫保护效果，该结果为进一步更好地利用SeM蛋白奠定了基础。

Abstract:

The aim of this study was to investigate the effects of the flagellin protein FliC of Salmonella abortus equi on immune response of Streptococcus equi subsp. equi SeM and provide basis for the development of a new effective strangle vaccine. The recombinant protein FliC was constructed, expressed and purified using affinity chromatography, and its immunogenicity was analyzed by Western blot. The purified FliC and SeM were used in-combination to immunize C57BL/6 mice and the serum antibody level, antibody subtype of immunized mice and challenge protection rate were investigated. SDS-PAGE result indicated that the expressed recombinant FliC protein was 52 kDa. The results of antibody typing showed that the main antibodies were mainly IgG1, and the challenge protection rate of FliC+SeM immunization group (87%) was higher than that of SeM immunization group (67%). The flagellin FliC of Salmonella abortus equi could enhance the immune efficacy of SeM and this result laid experimental foundation for the further utilization of SeM.

中图分类号:

赵亚南, 杨康, 张宝江, 李斌, 苏艳. 马流产沙门菌鞭毛蛋白FliC对马腺疫链球菌SeM蛋白免疫效应分析[J]. 畜牧兽医学报, 2018, 49(5): 1020-1026.

ZHAO Ya-nan, YANG Kang, ZHANG Bao-jiang, LI Bin, SU Yan. Immune Efficacy Analysis of Flagellin Protein FliC of Salmonella abortus equi on SeM of Streptococcus equi subsp. equi[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 1020-1026.

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